Johnson had placed Olly Barkley and Tom Rees on standby earlier in the week as the pair continued to receive treatment but today named the same starting squad which breathed life into England's campaign at Twickenham.
The last time England fielded the same 22 in consecutive Tests was against Australia and France in the quarter-finals and semi-finals of the 2007 World Cup.
Worsley and Flood took only a limited part in training today but Johnson is comfortable both players will be ready to face Scotland.
"We are confident both Joe and Toby will be fit," he said. "It is a short week but when you win it makes things easier.
"We have not been able to train too much this week but the session went well this morning and we are confident they will be fit."
Johnson reserved praise for England's full-back Delon Armitage, who has scored three tries during the championship and been shortlisted as one of the players of the Six Nations.
Armitage was also voted England's player of the autumn campaign and Johnson said: "Delon has been very good for us all season.
"He has taken his chance fantastically well for a guy who was not in the original 64-man squad. You find your way in how you can and he has taken his chance."
England team to play Scotland in the RBS 6 Nations at Twickenham on Saturday, March 21 (3:30pm):
D Armitage (London Irish); U Monye (Harlequins), M Tindall (Gloucester), R Flutey (Wasps), M Cueto (Sale Sharks); T Flood (Leicester), H Ellis (Leicester); A Sheridan (Sale Sharks), L Mears (Bath), P Vickery (Wasps), S Borthwick (Saracens, capt), S Shaw (Wasps), T Croft (Leicester), J Worsley (Wasps), N Easter (Harlequins).
Replacements: D Hartley (Northampton), J White (Leicester), J Haskell (Wasps), N Kennedy (London Irish), D Care (Harlequins), A Goode (Brive), M Tait (Sale Sharks).