Scrum-half Chris Cusiter has been ruled out of Scotland's November test series after fracturing his thumb.
The British & Irish Lions player needs surgery on the injury, which he sustained while playing for Perpignan, and will be out of action for about six weeks.
Scotland coach Frank Hadden has also lost centre Graeme Morrison and Rory Lawson, another scrum-half, from his training squad ahead of the Bank of Scotland Corporate Autumn Tests against New Zealand, South Africa and Canada.
Glasgow Warriors scrum-half Mark McMillan has been called into the pool for the training camp in Spain and flew out with the rest of the home-based players from Edinburgh Airport this morning.
Morrison, who scored the decisive try in Scotland's historic Test victory in Argentina in June, was deemed unfit to travel by Scotland team doctor James Robson.
Morrison suffered knee ligament damage in Glasgow's Magners League victory over Leinster and will undergo further assessment and treatment from the Warriors medical staff.
Lawson, who acted as understudy to captain Mike Blair on the summer tour of Argentina, suffered a rib cartilage injury playing for Gloucester yesterday and will see a specialist on Tuesday.
McMillan, 25, has never won a full cap but is a former under-21 captain and represented Scotland A during the summer.
There was better news for Hadden after Ospreys agreed to release winger Nikki Walker for the camp.
Walker will join up with the Scotland squad tomorrow provided he comes through unscathed from his club's match against Worcester.
As head coach Stuart Lancaster polishes the axe he will soon wield over his World Cup training squad, Exeter Chiefs coach Rob Baxter has backed Jack Nowell, Henry Slade and Luke Cowan-Dickie to be given a stay of execution.
Italy Under-20s held off a late Samoa blitz to retain their place in the World Rugby U20 Championship on Saturday and head coach Alessandro Troncon believes the experience will have served as a valuable lesson for his young troops.