But he starts his comeback just 48 hours before Wales coach Warren Gatland announces his squad for this season's RBS 6 Nations Championship.
Gatland will unveil the squad on Monday, the same day as Henson is due before Cardiff magistrates to answer a charge of disorderly conduct.
The charge arises from alleged incidents on a London to Cardiff train following Ospreys' win against Harlequins six weeks ago.
Henson teams up with Sonny Parker in midfield, with Wales back-row forward Ryan Jones captaining an Ospreys side which also includes fit-again wing Shane Williams.
The Ospreys must beat Premiership leaders Gloucester at the Liberty Stadium to keep alive their hopes of a quarter-final place.