Italy coach John Kirwan has drafted Benetton Treviso forward Silvio Orlando into his squad in preparation for the RBS 6 Nations clash against Scotland at Murrayfield on February 26.
Orlando deputises for experienced flanker Mauro Bergamasco, who misses out after suffering a fractured cheekbone in the 38-8 defeat at home to Wales on Saturday.
Full-back Roland De Marigny was another injury victim against Wales as he limped off the Stadio Flaminio pitch with knocks to his ankle and knee.
However, the Parma player is included in Kirwan's 25-man squad, along with Montpellier centre Cristian Stoica, who returns after being left out of the first two matches of the
The only real headache for Kirwan is whether Sergio Parisse will be available for the game at Murrayfield.
Italy's number eight will have to go before the Disciplinary Commission on Wednesday following his tackle on Wales player Stephen Jones during the match on Saturday.
The Treviso star has been provisionally included in the squad for the Scotland encounter.
Italy squad for RBS 6 Nations match with Scotland at Murrayfield on February 26:
M Barbini (Padova), Mirco Bergamasco (Stade Francais), M Bortolami (Narbonne), Martin Leandro Castrogiovanni (Calvisano), D Dal Maso (Treviso), R De Marigny (Parma), C Del Fava (Parma), S Dellape (Agen), P Griffen (Calvisano), G Intoppa (Calvisano), A Lo Cicero (L'Aquila), A Masi (Viadana), L Nitoglia (Calvisano), F Ongaro (Treviso), S Orlando (Treviso), L Orquera (Padova), S Parisse (Treviso), R Pedrazzi (Viadana), A Persico (Agen), S Perugini (Calvisano), W Pozzebon (Treviso), P Robertson (Viadana), M Savi (Viadana), C Stoica (Montpellier), A Troncon (Treviso).
England's preparations for the Autumn International series were potentially dealt a further blow as Maro Itoje was forced to undergo an x-ray after Saracens' European victory over Scarlets on Saturday night.