Centre Cristian Stoica was an absentee on Sunday as Italy's players gathered to prepare for next weekend's visit to Twickenham to play world champions England.
Stoica is mourning the death of a friend in a car accident and, with the funeral scheduled for Tuesday, the Italian coaching staff have not yet decided if the Romania-born player will make the trip to London.
The other 24 players called up were all present at the training camp in the Tuscany town of Tirrenia near Pisa.
Mirco Bergamasco and skipper Marco Bortolami linked up with the squad after flying in from London where they were involved in Saturday's tsunami aid match between the northern and southern hemispheres.
After lunch the players watched a video of the defeat by Scotland at Murrayfield before their first training session under the gaze of John Kirwan and his coaching team.
The 2017 RBS 6 Nations was watched by record numbers of fans
29 March 2017, 12:25pm
After an enthralling RBS 6 Nations Championship of twists, turns, and no small amount of drama on the last day, figures show the RBS 6 Nations is going from strength to strength, year on year, with no sign of slowing down.