Centre Cristian Stoica was an absentee on Sunday as Italy's players gathered to prepare for next weekend's visit to Twickenham to play world champions England.
Stoica is mourning the death of a friend in a car accident and, with the funeral scheduled for Tuesday, the Italian coaching staff have not yet decided if the Romania-born player will make the trip to London.
The other 24 players called up were all present at the training camp in the Tuscany town of Tirrenia near Pisa.
Mirco Bergamasco and skipper Marco Bortolami linked up with the squad after flying in from London where they were involved in Saturday's tsunami aid match between the northern and southern hemispheres.
After lunch the players watched a video of the defeat by Scotland at Murrayfield before their first training session under the gaze of John Kirwan and his coaching team.
As head coach Stuart Lancaster polishes the axe he will soon wield over his World Cup training squad, Exeter Chiefs coach Rob Baxter has backed Jack Nowell, Henry Slade and Luke Cowan-Dickie to be given a stay of execution.
Italy Under-20s held off a late Samoa blitz to retain their place in the World Rugby U20 Championship on Saturday and head coach Alessandro Troncon believes the experience will have served as a valuable lesson for his young troops.