After impressive wins over both Scotland and Ireland in this year's RBS 6 Nations, England come into their Twickenham clash with Wales on Sunday with their eyes on a Triple Crown.
The versatile Exeter Chiefs back-row Johnson looks set for a spot on the bench against Wales, England's conquerors 12 months ago in Cardiff, while Ben Morgan will start at No.8.
And after their strong showing in beating table toppers Ireland at Rugby HQ last time out, Lancaster has resisted the urge to make any other changes.
"The performance against Ireland has given us a lot of confidence going into this game against a high-quality Welsh team and we feel it's right to give the bulk of that 23 another opportunity," he said.
"Tom Johnson has trained with us throughout and we are looking forward to his contribution."
Leicester centre Manu Tuilagi joined England's training squad on Monday along with Marland Yarde but the London Irish wing will return to his club.
Lancaster said: "There is a lot of competition for places and to have Marland and Manu back training with us this week has been great.
"With Leicester and Wasps not involved in the LV= Cup semi-finals this weekend we are able to keep Matt Mullan to provide cover in the front row and also to give Manu more time to get up to speed with the team."