Jones, who captained Wales in their last three matches, came off in the second half of the 28-18 win over Scotland after injuring his shoulder and Justin Tipuric, Andrew Coombs and Aaron Shingler will battle it out to replace him in the back row.
The stage is set for a dramatic climax on Saturday, with Wales bouncing back from their initial defeat to Ireland - an eighth successive defeat - to win three straight away from home and set up a decider in Cardiff.
"The fact that people wrote us off after the first game, to come back with three away wins, the boys were extremely pleased with that," said centre Jonathan Davies.
"We understood that we could cause problems in this Championship and do very well."
England's narrow victory over Italy at Twickenham on Sunday means that a victory by eight points will guarantee Wales a second successive title, after last year's Grand Slam, their third in eight years.
England will need to bounce back at the Millennium Stadium in what is sure to be a highly-charged affair but Davies sees the pressure Wales will face as a positive thing.
"When the pressure is put on us we just thrive on it," added the 24 year-old Scarlets centre.
"This group of players are used to a big-game environment.
"We've been in these big games before and we have experience in them. We have experience from last year's Grand Slam and the semi-final in the World Cup.
"It's an exciting week ahead and we just have to make sure that we do everything we can to be ready for it.
"We know what to expect and we are just going about our business normally and keeping our heads down.
"We need to trust our defensive pattern and make sure that we keep doing what we have been doing in the last three games.
"Cardiff is the best place to play rugby in the world. I am sure there will be an amazing atmosphere at the stadium."