After a year playing in Japan and then with the Highlanders in the Super 15, Haskell will return to the Premiership next season with Wasps but that move could not prevent his demotion into the Saxons squad.
The 27-year-old started the final Test of the summer series against South Africa that was drawn 14-14, with 26 members of that touring party making up the senior squad.
With Lee Mears and Charlie Hodgson retiring from international rugby, the new faces are Leicester trio Anthony Allen, Geoff Parling and Thomas Waldrom along with Danny Care, Alex Goode, Tom Johnson and Jonathan Joseph.
However David Strettle, Paul Doran-Jones and Ugo Monye are also demoted to the Saxons while there's no room in either squad for Matt Banahan, Delon Armitage or Andy Saull, Saracens' outstanding openside flanker.
But Lancaster was keen to emphasise the door was not permanently shut on those who have been left out of the senior squad.
He said: "We spent a long time deciding on these 64 and there were some tough decisions to make. Selecting players at this stage gives us an early chance to sit down and assess the tour and look forward to next season.
"However, experience tells us that form and fitness will play a major role in selection prior to the QBE Internationals in November and December and any of the players in the Senior and Saxons squads could easily be involved.
"We saw this in January when Geoff Parling came in as an injury replacement for Louis Deacon and has played every Test since - there is always movement which opens the door for players and I am sure next season will be no different.
"The great thing is that we have got competition across the board and strength in depth developing in many positions with players who will be around for the next few years.
"There are also some exciting players just on the cusp of the squads such as Tom Homer, Charlie Matthews, Tom Heathcote and Elliot Daly, who we are really looking forward to seeing develop in the next 12 months and beyond."
England elite squad: Mouritz Botha (Saracens), Alex Corbisiero (London Irish), Dan Cole (Leicester Tigers), Tom Croft (Leicester Tigers), Phil Dowson (Northampton Saints), Dylan Hartley (Northampton Saints), Tom Johnson (Exeter Chiefs) Courtney Lawes (Northampton Saints), Joe Marler (Harlequins) Ben Morgan (Gloucester), Tom Palmer (London Wasps), Geoff Parling (Leicester Tigers), Chris Robshaw (Harlequins), Matt Stevens (Saracens), Thomas Waldrom (Leicester Tigers) Rob Webber (Bath), Tom Wood (Northampton Saints), Anthony Allen (Leicester Tigers), Chris Ashton (Saracens), Brad Barritt (Saracens), Mike Brown (Harlequins), Danny Care (Harlequins), Lee Dickson (Northampton Saints), Owen Farrell (Saracens), Toby Flood (Leicester Tigers), Ben Foden (Northampton Saints), Alex Goode (Saracens),Jonathan Joseph (London Irish), Charlie Sharples (Gloucester), Manusamoa Tuilagi (Leicester Tigers), Jordan Turner-Hall (Harlequins), Ben Youngs (Leicester Tigers).
England Saxons: Nathan Catt (Bath), Calum Clark (Northampton Saints), Louis Deacon (Leicester Tigers), Paul Doran Jones (Northampton Saints), Carl Fearns (Bath), James Gaskell (Sale Sharks), Jamie Gibson (London Irish), Joe Gray (Harlequins), James Haskell (London Wasps), Graham Kitchener (Leicester Tigers), Matt Kvesic (Worcester Warriors), Joe Launchbury (London Wasps), Matt Mullan (Worcester Warriors), David Paice (London Irish), George Robson (Harlequins), Henry Thomas (Sale Sharks), David Wilson (Bath), Tom Youngs (Leicester Tigers), Nick Abendanon (Bath), Miles Benjamin (Leicester Tigers), Tom Biggs (Bath), Freddie Burns (Gloucester), George Ford (Leicester Tigers), George Lowe (Harlequins), Jonny May (Gloucester), Rob Miller (Sale Sharks), Ugo Monye (Harlequins), Joe Simpson (London Wasps), David Strettle (Saracens), Billy Twelvetrees (Gloucester), Christian Wade (London Wasps), Richard Wigglesworth (Saracens).