Jason Robinson and Neil Back have become the latest victims of England's RBS 6 Nations injury problems.
Both players went off during the 26-9 victory over Wales in Cardiff and could struggle to make England's March 9 appointment with Italy at Twickenham.
Full-back Robinson suffered a twisted knee, while flanker Back hurt his calf - giving England boss Clive Woodward further fitness worries during a campaign already riddled by injuries.
Props Jason Leonard (hamstring), Julian White (knee) and Phil Vickery (back) are all unlikely to feature in the remaining games against Italy, Scotland and Ireland.
But fly-half Jonny Wilkinson and wing Dan Luger, who also departed the Millennium Stadium action early, should be fit for Italy.
"Jason Robinson and Neil Back are concerns," admitted Woodward, whose lengthy casualty list additionally includes the likes of Matt Dawson, Austin Healey and Iain Balshaw.
Wasps back Josh Lewsey and Leicester's Tim Stimpson will come into contention for the number 15 jersey should Robinson be ruled out against Italy, with Joe Worsley and Alex Sanderson providing clear alternatives in Back's possible absence.
But it looks as if Wilkinson, England's key man with 36 of his team's 51 points in the championship to date, will make the Italy encounter after suffering a knee problem.
"I came off as a precaution at the end, and it feels a lot better now," said Wilkinson.
Conor O'Shea will lead his side to matches against Fiji and Australia
30 March 2017, 2:36pm
After a disappointing RBS 6 Nations campaign for the Azzurri - losing all five of their Championship matches and picking up their 12th Wooden Spoon - they will be licking their wounds ahead of their summer tour Down Under.
The 2017 RBS 6 Nations was watched by record numbers of fans
29 March 2017, 12:25pm
After an enthralling RBS 6 Nations Championship of twists, turns, and no small amount of drama on the last day, figures show the RBS 6 Nations is going from strength to strength, year on year, with no sign of slowing down.