Billy Twelvetrees started all five of England's RBS 6 Nations games this year
Billy Twelvetrees' participation in England's summer tour to New Zealand has been plunged into doubt after Gloucester director of rugby Nigel Davies expressed his concerns over his ankle injury.
The 25-year-old, who started at centre in all five of England's RBS 6 Nations match this year, took a blow to his ankle injury as Gloucester secured a 38-30 win over London Irish.
The extent of the injury is due to be known soon but Gloucester director of rugby Davies did hint at a concern that the centre might be out for a significant period of time.
"We all know how committed Billy is as a player and for him to come off , especially in this game with a lot of emotion going on, says a lot but we will have to wait and see," said Davies.
"It's an ankle problem but with all of these things it's very difficult to give a formal assessment. We will monitor the situation over the next 48-hours and in that period we will have a scan to determine the amount of damage.
"It's not great for him but until we have a clearer picture we can't say how serious it is."
Andy Farrell admits the process of selecting England's 50-man training squad ahead of the World Cup was rigorous - but the backs and defence coach also revealed how tough it was to leave some stars out.
Wales head coach Warren Gatland says he hopes to have Jonathan Davies back and firing on all cylinders for the 2016 RBS 6 Nations after the centre ruptured his anterior cruciate ligament over the weekend.