Nick de Luca faces a race against time to be fit for the RBS 6 Nations clash with England at Murrayfield next Saturday.
The 24-year-old centre injured his right ankle in Edinburgh's 10-10 draw in the Magners League with Newport Gwent Dragons.
Initial medical assessments suggest the Dumfries-born back, one of Edinburgh's few internationals who played in the match at Rodney Parade, has sprained the ankle.
De Luca started Scotland first two matches, against France and Wales, before coming off the bench in the defeat against Ireland last weekend.
Should he miss out, it could offer a first Test opportunity to Ben Cairns, who scored a try in his first game as captain of Andy Robinson's side.
The former England coach said: "He took his try very well and Ben has certainly developed over the past three or four months.
"We managed the game well defensively in the second half but what was disappointing was that we didn't score points ourselves."
Meanwhile, Dan Parks kicked all Glasgow's points as they defeated the Ospreys 9-6 - but Scotland lock Ally Kellock was taken off with a leg muscle strain.
He must also be now a doubt for Frank Hadden ahead of the clash with England, although Nikki Walker came through unscathed for the Welsh side.